xfect kit Search Results


xfect kit  (TaKaRa)
96
TaKaRa xfect kit
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect protein transfection kit  (TaKaRa)
93
TaKaRa xfect protein transfection kit
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Protein Transfection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect protein transfection kit - by Bioz Stars, 2026-03
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xfect transfection kit  (TaKaRa)
95
TaKaRa xfect transfection kit
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Transfection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect transfection kit - by Bioz Stars, 2026-03
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xfect tm transfection reagent kit  (TaKaRa)
97
TaKaRa xfect tm transfection reagent kit
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Tm Transfection Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect tm transfection reagent kit - by Bioz Stars, 2026-03
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xfect transfection reagent  (TaKaRa)
96
TaKaRa xfect transfection reagent
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Transfection Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect transfection reagent - by Bioz Stars, 2026-03
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xfect mesc transfection reagent takara bio  (TaKaRa)
94
TaKaRa xfect mesc transfection reagent takara bio
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Mesc Transfection Reagent Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xfect sirna transfection kit  (TaKaRa)
90
TaKaRa xfect sirna transfection kit
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Xfect Sirna Transfection Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biomasher standard  (TaKaRa)
95
TaKaRa biomasher standard
( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP <t>tagged</t> <t>mHtt-Ex1</t> (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the <t>Xfect</t> kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013
Biomasher Standard, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP tagged mHtt-Ex1 (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the Xfect kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013

Journal: eLife

Article Title: Control of the structural landscape and neuronal proteotoxicity of mutant Huntingtin by domains flanking the polyQ tract

doi: 10.7554/eLife.18065

Figure Lengend Snippet: ( A ) General schematic of trans N17 addition experiments to ∆N aggregation reaction to determine how N17 impacts the balance between stable, ∆N oligomers and amyloid fibrillar aggregates. ( B ) 12.5x excess of N17 peptide was added when ∆N aggregation was initiated. Then 60 hr after initiation of aggregation, the fibers were analyzed by cryo-EM. N17-seeded ∆N aggregates become more bundled and more resemble the morphology of Ex1 aggregates . ( C ) 12.5x or 2.5x excess of N17 peptide was added in trans when ∆N aggregation was initiated. The impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. Trans addition of N17 promotes disappearance of these stable ∆N oligomers from the AGE gel. ( D ) 12.5x excess of N17 peptide was added in trans 7 hr after initiation of ∆N aggregation, allowing for the formation of ∆N oligomers before N17 addition. Impact of N17 peptide on ∆N oligomers was analyzed by 0.1% SDS-AGE gels and immunoprobed for the C-terminal S-tag. ( E ) Schematic of in vivo N17 addition experiment: ST14a striatal-derived neurons were transfected with the C-terminally GFP tagged mHtt-Ex1 (mHtt-Ex1-GFP). 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the Xfect kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation. After 10 hr, resulting cells containing GFP fluorescent puncta are counted. ( F ) Fluorescence microscopy of ST14a striatal neurons transfected with mHtt-Ex1-GFP and N17 variant peptides (left) and quantification of cells containing puncta (right). Data are mean ± SEM of three independent experiments with at least 200 cells counted in each condition. Scale bar is 20 µm. *p<0.05. DOI: http://dx.doi.org/10.7554/eLife.18065.013

Article Snippet: 10 hr after transfection, when expressed mHtt protein is still completely soluble, cells are protein transfected using the Xfect kit (Clontech) with N17 or a mutant N17 peptide (NA) that inhibits aggregation.

Techniques: Cryo-EM Sample Prep, In Vivo, Derivative Assay, Transfection, Mutagenesis, Fluorescence, Microscopy, Variant Assay